Spiroplasma citri Spiralin Acts In Vitro as a Lectin Binding to Glycoproteins from Its Insect Vector Circulifer haematoceps
نویسندگان
چکیده
منابع مشابه
Spiralin is not essential for helicity, motility, or pathogenicity but is required for efficient transmission of Spiroplasma citri by its leafhopper vector Circulifer haematoceps.
Spiralin is the most abundant protein at the surface of the plant pathogenic mollicute Spiroplasma citri and hence might play a role in the interactions of the spiroplasma with its host plant and/or its insect vector. To study spiralin function, mutants were produced by inactivating the spiralin gene through homologous recombination. A spiralin-green fluorescent protein (GFP) translational fusi...
متن کاملThe Repetitive Domain of ScARP3d Triggers Entry of Spiroplasma citri into Cultured Cells of the Vector Circulifer haematoceps
Spiroplasma citri is a plant pathogenic mollicute transmitted by the leafhopper vector Circulifer haematoceps. Successful transmission requires the spiroplasmas to cross the intestinal epithelium and salivary gland barriers through endocytosis mediated by receptor-ligand interactions. To characterize these interactions we studied the adhesion and invasion capabilities of a S. citri mutant using...
متن کاملجدایه جدید Spiroplasma citri جدا شده از زنجرک Circulifer haematoceps در استان فارس
جدایه‌های Spiroplasma citri براساس تحرک الکتروفورزی پروتئین و RFLP ژن اسپیرالین به پنج گروه تقسیم شده‌اند (Foissac et al. 1996). در این گزارش دو جدایه جدید S. citri که واجدشرایط برای پایه‌گذاری گروه ششم هستند معرفی می‌گردد....
متن کاملnovel isolate of spiroplasma citri from leafhopper circulifer haematoceps from fars province
strains of spiroplasma citri have been classified into five groups based on electrophoretic mobility (em) of spiralin protein and rflp analysis of spiralin gene (foissac et al. 1996). in the present study, two isolates of s. citri qualifying for formation of a sixth group are reported. infection of sesame plants by s. citri has been reported previously (salehi and izadpanah 2002). samples of ve...
متن کاملDisruption of a gene predicted to encode a solute binding protein of an ABC transporter reduces transmission of Spiroplasma citri by the leafhopper Circulifer haematoceps.
Spiroplasma citri is transmitted from plant to plant by phloem-feeding leafhoppers. In an attempt to identify mechanisms involved in transmission, mutants of S. citri affected in their transmission must be available. For this purpose, transposon (Tn4001) mutagenesis was used to produce mutants which have been screened for their ability to be transmitted by the leafhopper vector Circulifer haema...
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ژورنال
عنوان ژورنال: Phytopathology®
سال: 2005
ISSN: 0031-949X,1943-7684
DOI: 10.1094/phyto-95-0541